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                                   BICINE 生物緩沖液

Sigma B8660 assay≥99% (titration)
Product Description
分子式: C6H13NO4
分子量: 163.2
CAS Number: 150-25-4
pKa: 8.3 (0.1 M, 25 °C)1
Melting Point: 190 - 192 °C2
Useful pH range: 7.6 - 9.0
別名: N,N-bis(2-hydroxyethyl)glycine,
di(hydroxyethyl)glycine,
N,N-bis(hydroxyethyl)aminoacetic
acid

    Trace elemental analyses have been performed on the SigmaUltra bicine. The Certificate of Analysis provides lot-specific results. SigmaUltra bicine is for applications which require tight control of elemental content.

    Bicine is a zwitterionic buffer used in biochemistry and molecular biology research. Originally prepared in the 1920's, it later became one of the Good buffers that were studied in the 1960's to provide buffers in the pH range of 6.15 - 8.35 for wide applicability to biochemical studies.

    The use of bicine in a thin layer ion exchange chromatography method for protein resolution has been published.3 Bicine has been utilized in peptide and protein crystallization.4,5,6 A kinetic study of a quaternary transition-state analogue complex of creatine kinase used bicine in the reaction buffer.7 A multiphasic buffer system for SDS-PAGE of proteins and peptides that incorporates bicine has been described.8

Precautions and Disclaimer
    For Laboratory Use Only. Not for drug, household or other uses.

Preparation Instructions
    This product is soluble in water (330 mg/ml), yielding a clear, colorless solution.   

現貨供應:270元/25g     540元/100g

 

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